| Clay Aiken | Brother Maynard | (Jan 18, 2008-May 4, 2008) |
| Guard 1 | (Jan 18, 2008-May 4, 2008) | |
| Sir Robin | (Jan 18, 2008-May 4, 2008) | |
| Brother Maynard | (Sep 19, 2008-Jan 4, 2009) | |
| Guard 1 | (Sep 19, 2008-Jan 4, 2009) | |
| Sir Robin | (Sep 19, 2008-Jan 4, 2009) | |
| Hank Azaria | Sir Lancelot | (Dec 2, 2005-Apr 2, 2006) |
| Simon Russell Beale | King Arthur | (Dec 20, 2005 - ?) |
| John Bolton During Tim Curry's vacation | King Arthur | (Jul 22, 2005-Aug 7, 2005) |
| Jonathan Brody | Ensemble | |
| French Guard | ||
| Lewis Cleale | Prince Herbert's Father | (Jul 5, 2006 - ?) |
| Sir Dennis Galahad | (Jul 5, 2006 - ?) | |
| The Black Knight | (Jul 5, 2006 - ?) | |
| Stephen Collins | King Arthur | (Jun 24, 2008 - ?) |
| Kevin Covert | Monk | |
| Kevin Crewell | Ensemble | |
| Monk | ||
| Sir Not Appearing | ||
| Matthew Crowle | Ensemble | |
| Nun | ||
| Merle Dandridge | The Lady of the Lake | (Sep 2, 2008 - ?) |
| Jeff Dattilo | Concorde | |
| Dennis's Mother | ||
| Sir Bedevere | ||
| Bradley Dean | Prince Herbert's Father | (Mar 25, 2008 - ?) |
| Sir Dennis Galahad | (Mar 25, 2008 - ?) | |
| The Black Knight | (Mar 25, 2008 - ?) | |
| Tom Deckman | French Guard | |
| Historian | ||
| Minstrel | ||
| Not Dead Fred | ||
| Prince Herbert | ||
| Wally Dunn | Sir Bedevere | (Jun 17, 2008 - ?) |
| Andrew Fitch | Ensemble | |
| Asmeret Ghebremichael | Ensemble | |
| Minstrel | ||
| Harry Groener | King Arthur | (Apr 25, 2006 - ?) |
| Jonathan Hadary | King Arthur | (Oct 31, 2006 - ?) |
| Amy Heggins | Ensemble | |
| David Hibbard | Guard 2 | |
| Mayor | ||
| Patsy | ||
| Patsy | (Sep 16, 2008 - ?) | |
| Chris Hoch | Sir Lancelot | (Oct 3, 2006 - ?) |
| Rick Holmes | Knight of Ni | |
| Sir Lancelot | ||
| The French Taunter | ||
| Tim the Enchanter | ||
| Steve Kazee | Knight of Ni | (Apr 4, 2006-Oct 1, 2006) |
| Sir Lancelot | (Apr 4, 2006-Oct 1, 2006) | |
| The French Taunter | (Apr 4, 2006-Oct 1, 2006) | |
| Tim the Enchanter | (Apr 4, 2006-Oct 1, 2006) | |
| Lauren Kennedy | The Lady of the Lake | (Dec 20, 2005 - ?) |
| Kristie Kerwin | Ensemble | |
| Jeffrey Kuhn | Concorde | |
| Dennis's Mother | ||
| Sir Bedevere | ||
| Drew Lachey | Guard 2 | (Jun 24, 2008 - ?) |
| Mayor | (Jun 24, 2008 - ?) | |
| Patsy | (Jun 24, 2008 - ?) | |
| Gavin Lodge | Ensemble | |
| French Guard | ||
| Minstrel | ||
| Lindsay Lopez | Ensemble | |
| Minstrel | ||
| Joanne Manning | Ensemble | (Feb 21, 2006-Mar 12, 2006) |
| Brian J. Marcum | Ensemble | |
| Sir Bors | ||
| Minstrel | ||
| Marin Mazzie | The Lady of the Lake | (Oct 31, 2006-Jan 13, 2008) |
| The Lady of the Lake | (Jun 17, 2008-Aug 31, 2008) | |
| Martin Moran | Brother Maynard | (Apr 4, 2006 - ?) |
| Guard 1 | (Apr 4, 2006 - ?) | |
| Sir Robin | (Apr 4, 2006 - ?) | |
| Brother Maynard | (Jan 6, 2009-Jan 11, 2009) | |
| Guard 1 | (Jan 6, 2009-Jan 11, 2009) | |
| Sir Robin | (Jan 6, 2009-Jan 11, 2009) | |
| Brad Oscar | Concorde | (Dec 18, 2007 - ?) |
| Dennis's Mother | (Dec 18, 2007 - ?) | |
| Sir Bedevere | (Dec 18, 2007 - ?) | |
| Robert Petkoff Returns after playing Sir Robin in the National Tour | Sir Robin | (May 6, 2008 - ?) |
| Steve Rosen | Concorde | (Apr 29, 2008 - ?) |
| Dennis's Mother | (Apr 29, 2008 - ?) | |
| Sir Bedevere | (Apr 29, 2008 - ?) | |
| Brian Shepard | Nun | |
| Michael Siberry | King Arthur | (Sep 16, 2008 - ?) |
| Christopher Sieber | Prince Herbert's Father | (May 1, 2007-Mar 23, 2008) |
| Sir Dennis Galahad | (May 1, 2007-Mar 23, 2008) | |
| The Black Knight | (May 1, 2007-Mar 23, 2008) | |
| Vanessa Sonon | Ensemble | |
| Alan Tudyk (during Hank Azaria's hiatus) | Sir Lancelot | (Jun 7, 2005-Dec 1, 2005) |
| Hannah Waddingham | The Lady of the Lake | (Jan 13, 2008 - ?) |
| Patrick Wetzel | Ensemble | |
| Monk | ||
| Sir Not Appearing | ||
| Brandi Wooten | Ensemble |